#NEXT GENERATION SEQUENCE ANALYSIS HOW TO#

#NEXT GENERATION SEQUENCE ANALYSIS HOW TO#

How to remove sequencing reads with low quality, artifacts, duplication and contamination.How to perform basic operations with NGS data.How to evaluate the quality of raw NGS data.If in doubt, do not hesitate to contact us.If, on the other hand, you have no knowledge of NGS at all, we recommend that you first take the theoretical Introduction to Next-Generation Sequencing course.

If you already have some experience in NGS data analysis and want to revive and possibly deepen it (regardless of the specific procedures for a particular NGS application), this event is also for you.We recommend going through one of the freely available guides. Knowledge of BASH and the terminal is not required, but is an advantage. A necessary prerequisite is computer literacy and basic knowledge of molecular biology (DNA, RNA, gene expression, PCR).It is perfectly suitable for complete beginners in the field of data analysis, no previous experience with sequencing data analysis is required. The event is intended for biologists or bioinformaticians interested in analyzing the results of Next-Generation sequencing.After this three-day workshop, you will not become a bioinformatician, but you will be well on your way to becoming a qualified partner for bioinformaticians in your team. You will get acquainted with various formats of NGS data, you will learn about specific advantages and disadvantages of used sequencing technologies and how to deal with them, and you will understand the background of individual processes used to modify NGS data. The result should be a perfect mastery of the process, the output of which is a set of high quality data that can be used for subsequent analysis. Its goal is to learn to perform the basic steps of NGS data analysis, including manipulation of raw data, quality control, removal of low quality sequences, sequence adapters, artifacts or other contamination. Our workshop will allow you to understand the basics of NGS data processing. This is also a serious problem for experienced professionals in life sciences who have never studied NGS, simply because it is too fresh, and suddenly need to adapt to completely new tools, workflows and scientific terminology, not to analyze NGS data themselves - after all, that is why we have bioinformaticians, but to be able to ask the right questions and understand the results. Due to their quantity and especially their complexity, NGS data are typically processed by qualified bioinformaticians. Today, Next-Generation sequencing technologies (NGS) are an essential part of almost all life sciences research and diagnostics.

Next generation sequence analysis